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I-9: Important Components in Human Embryo Culture Media

Jacobsson AH Vitrolife Sweden AB, Goteborg, Sweden

Culture Media and Incubation

The test is applied to substances, preparations, or articles METHOD which, according to the Pharmacopeia, are required to be sterile. However, a satisfactory result only indicates that no con1. Seed the indicator cell culture at a suitable density taminating microorganism has been found in the sample ex(for example, 2 × 104 to 2 × 105 cells/mL, 4 × 103 to amined under the conditions of the test...

Culture Media and Incubation

The test is applied to substances, preparations, or articles METHOD which, according to the Pharmacopeia, are required to be sterile. However, a satisfactory result only indicates that no con1. Seed the indicator cell culture at a suitable density taminating microorganism has been found in the sample ex(for example, 2 × 104 to 2 × 105 cells/mL, 4 × 103 to amined under the conditions of the test...

Culture Media for Brucella.

Tissue Culture Media

1. Human placental cord serum contains a factor or factors necessary for active growth of fibroblasts in tissue culture and a factor which prevents the appearance of a characteristic toxicity. 2. The growth-promoting factor(s) is stable to heating to 60 degrees C. for 30 minutes and is associated with the euglobulin fraction of the serum. 3. Extraction of dried cord serum with benzene, chlorofo...